In The Bourne Ultimatum (IMDB), the CIA needs to hack the mail server of a newspaper (The Guardian UK) to read the email of a reporter they assassinated. So they turn to Nmap and its new official GUI Zenmap to hack the mail server! Nmap reports that the mail server is running SSH 3.9p1, Posfix smtpd, and a name server (presumably bind). They also make substantial use of Bash, the Bourne-again shell. Congratulations to Roger Chui for being the first to spot this. He also sent a scene transcript and the following HD screen shots:
Other movies which have used Nmap include: The Matrix Reloaded, The Listening, Battle Royale, and, uhh, HaXXXor: No Longer Floppy (NSFW). Screens shots of Nmap in all of these movies are available here. Nmap is becomming quite the movie star!